Episode #24 - How to pellet cells after permeabilization? Intracellular staining to measure targets of interest is a powerful extension of traditional surface immunophenotyping, but it is not without some issues… (Read more below.) Care must be taken with permeabilized cells, as they do not pellet as well as unfixed cells. They tend to pull off the tube wall when the liquid is aspirated. It is important to mark the side that the sample is expected to pellet against. Permeabilized cells are also